The family of SuperLumia ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. SuperLumia ECL Plus chemiluminescent HRP substrate reagent is specially optimized for femtogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate’s intense signal output and duration enables detection of femtogram amounts of antigen with photographic or other imaging methods perfectly well.
For best results, it is essential to optimize all system components including sample amount, antibody concentrations and the membrane and blocking reagents. SuperLumia ECL Reagent is extremely sensitive and requires much less sample and antibodies than most commercially available substrates.
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