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            當前位置 : NEB >>> NEB/Q5? Hot Start High-Fidelity DNA Polymerase/500 units/M0493L
            NEB/Q5? Hot Start High-Fidelity DNA Polymerase/500 units/M0493L
            • NEB/Q5? Hot Start High-Fidelity DNA Polymerase/500 units/M0493L

            NEB/Q5? Hot Start High-Fidelity DNA Polymerase/500 units/M0493L

            價格: 試用 市場價: 0.00

            貨號: M0493L
            品牌: NEB
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              • Q5® Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.

                M0493
                Amplification of a variety of human genomic amplicons fromlow to high GC content using Q5 Hot Start High-Fidelity DNA Polymerase. Allreactions were set up at room temperature, conducted using 30 cycles ofamplification and visualized by microfluidic LabChip® analysis.

                Product Source

                An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
                This product is related to the following categories:
                Q5® High-Fidelity DNA Polymerases Products
                This product can be used in the following applications:
                Hot Start PCR,
                Long Range PCR,
                Genome Editing Applications,
                Fast PCR,
                High-Fidelity PCR,
                Multiplex PCR,
                Specialty PCR,
                Routine PCR,
                DNA Amplification, PCR & qPCR,
                PCR,
                Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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