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NEB/Phusion? High-Fidelity DNA Polymerase/100 units/M0530S

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貨號: M0530S

品牌: NEB

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- High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
  Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl₂ (1.5 mM at the final [1X] reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl₂ solutions.
  Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995.
  A 3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacturer"s recommended conditions. Extension times are indicated (in minutes). Ladder L is a 1 kb DNA Ladder (NEB# N3232).
  Product Source
  AnE. colistrain that carries the Phusion DNA Polymerase gene.
  This product is related to the following categories:
  Phusion® High-Fidelity DNA Polymerases Products
  This product can be used in the following applications:
  Gibson Assembly®,
  Long Range PCR,
  Fast PCR,
  High-Fidelity PCR,
  Multiplex PCR,
  Specialty PCR,
  Routine PCR,
  PCR
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