當前位置： NEB >>> NEB/NEBNext? High-Fidelity 2X PCR Master Mix/M0541S/250 reactions

NEB/NEBNext? High-Fidelity 2X PCR Master Mix/M0541S/250 reactions

價格: ￥4320.00 市場價: 7200.00

打印產品信息

貨號: M0541S-250reactions

品牌: NEB

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即購買加入購物車

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

- Description:
  
  ?
  TheNEBNextHigh-Fidelity2XPCRMasterMixisspecificallyoptimizedfortherobust,high-fidelityamplificationofnext-generationsequencing(NGS)libraries,regardlessofGCcontent.Thepolymerasecomponentofthemastermix,Q5??High-FidelityDNAPolymerase,isanovelThermostableDNApolymerasethatpossesses3′→5′exonucleaseactivity,andisfusedtoaprocessivity-enhancingSso7ddomain.Q5High-FidelityDNAPolymerasealsohasanultra-lowerrorrate(>100-foldlowerthanthatofTaqDNAPolymeraseand6-foldlowerthanthatofPyrococcusfuriosus(Pfu)DNAPolymerase).Thebuffercomponentofthemastermixhasbeenoptimizedforrobustamplification,evenwithGC-richamplicons.
  
  Figure1:ComparativeAnalysisofDifferentDNAPolymeraseswithKnownLowCoverageRegions.
  IndexedlibrarieswerepreparedfromhumanIMR90DNAandsplitintoindividualsamplesforlibraryamplification.Amplificationwasperformedusing8cyclesofPCRwithPhusionHigh-FidelityDNAPolymerase,KAPAHiFi?HotStartReadyMixorNEBNext?High-Fidelity2XPCRMasterMix.LibrariesweresequencedonanIlluminaHiSeq?2000.Tocorrectforslightdifferencesinthenumberofalignedreadsfromeachlibrary,180millionreadswererandomlyextractedfromeachdataset,representinganaveragecoverageof~6X.Thenumberofreadsoverlappingdistinctlowcoverageregionsofthehumangenome(Airdet.al.GenomeBIOLOGy,2011)areshownforeachlibrary.TheNEBNextHigh-Fidelity2XPCRMasterMixgivesthemostoptimalperformanceofthethreeenzymes/mastermixestested.
  Figure2:FidelityComparisonsofDifferentDNAPolymerases.
  Fidelitymeasurementsof?Taq?DNAPolymerase(inStandard?Taq?Buffer),KAPAHiFiHotStartReadyMixandNEBNextHigh-Fidelity2XPCRMasterMixweremeasuredside-by-sideinaPCR-basedmutationscreeningassayusingalacZmethodmodifiedfromKermekchievetal.,2003.Values(n≥2)areexpressedrelativetoKAPAHiFiHotStartReadyMix.TheQ5High-FidelityPolymeraseintheNEBNextHigh-Fidelity2XPCRMasterMixhasaleveloffidelity>10XhigherthanthatofthepolymeraseintheKAPAHiFiHotStartReadyMix.
  Figure3:ComparativeAnalysisofDifferentDNAPolymeraseswithGenomesofVarying%GC.
  LibrariesofH.influenza,R.palustrisorhumangenomicDNAwereamplifiedusingNEBNextHigh-Fidelity2XPCRMasterMix,PhusionHigh-FidelityPCRMasterMixwithHFBufferorKAPAHiFiHotStartPCRReadyMix,andsequencedonanIllumina?HiSeq2000.GCcoverageplotsweregenerated,with%GCcontentof100bpwindowsontheXaxis.Normalizedcoverageisindicatedbythebluecircles,windowsatGC%indicatedbytheredlines,andbasequalityatGC%indicatedbythegreenline.NEBNextHigh-Fidelity2XPCRMasterMixprovidessubstantiallyreducedbiasonallthreegenomicDNAsamples.
  Figure4:ComparativeAnalysisofDifferentDNAPolymerasesatVaryingGC%s.
  AmplifiedlibrariesofhumangenomicDNAweregeneratedusingindexprimersandNEBNextHigh-Fidelity2XPCRMasterMix,Phusion?High-FidelityPCRMasterMixwithHFBufferorKAPAHiFiHotStartReadyMix,andsequencedonanIllumina?HiSeq?2000.AnequalnumberofreadsfromeachdatasetwererandomlyselectedandthepercentageofreadswasplottedagainstGCcontent.NEBNextHigh-Fidelity2XPCRMasterMixandKAPAHiFHotStartReadyMixalignedcloselytotheexpectedreadfrequencies(shadedgrey),whilePhusiondidnot.
  ProductSource
  AnE.colistrainthatcarriestheQ5High-FidelityDNAPolymerasegene.
  ReactionVolumeDefinition
  NEBNextHigh-Fidelity2XPCRMasterMix,DNAtemplateand0.5μMto1.25μMprimers(dependingonsampleinput)inatotalreactionvolumeof50μl.
  Notes:
  Toensureoptimalperformance,themastermixshouldbethawedandresUSPendedpriortouse.StABIlitytestingusingupto20freeze/thawcycleshasshownnonegativeeffectonmastermixperformance.TheNEBNextHigh-Fidelity2XPCRMasterMixmaybeliquidat-20°C.
售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Parafilm M封口膜/PM996/4x125/盒
￥0.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥850.00
ThermoFisherQ32856Qubit? Assay Tubes現貨
￥590.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00

NEB/NEBNext? High-Fidelity 2X PCR Master Mix/M0541S/250 reactions

Description:

ProductSource

ReactionVolumeDefinition

Notes:

客戶服務

何為188

關注188微信公眾號