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Millipore/MAB079-1 | Anti-S-100 Protein Antibody, clone 15E2E2/MAB079-1/100 µL@ 1 mg/mL concentation

價格: ￥3444.00 市場價: 5740.00

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貨號: MAB079-1

品牌: Millipore

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Description
CatalogueNumber	MAB079-1
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-S-100ProteinAntibody,clone15E2E2
AlternateNames	CalciumBindingProteinA1/B1
BackgroundInformation	S-100proteinisamemberoftheEF-handcalcium-bindingproteinsuperfamilythatiswidelydistributedandconservedinthecentralnervoussystemofvertebrates.Theproteinexistsinbothheterodimericandhomodimericforms.ThetwosubunitsofS-100proteinaretheproductsofseparategenesthataredifferentiallyexpressedbyvariouscells.ThebetasubunitispresentinallS-100positivecellsandtumors.Incontrast,thealphasubunitisdetectableonlyinheart,skeletalmuscleandbrain.Inadditiontoitscalciumbindingproperties,S-100proteinhasahigh-affinitybindingsiteforzincandisinvolvedintheregulationofproteinphosphorylationinthebrain.Theproteinapparentlyplaysaroleincelldifferentiationandgrowth,cytoskeletalstructureandfunction,andhasbeenimplicatedinneuropathologicaldiseases.

ProductInformation
Format	Purified
Control	PositiveControlTissue:Melanoma
Presentation	PurifiedmousemonoclonalIgG2aκinbuffercontainingPBS,pH7.6,1%BSAand0.09%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8oCfromdateofreceipt.

Applications
Application	Anti-S-100ProteinAntibody,clone15E2E2isanantibodyagainstS-100ProteinforuseinIH,IH(P),IP&WB.
KeyApplications	Immunohistochemistry Immunohistochemistry(Paraffin) Immunoprecipitation WesternBlotting
ApplicationNotes	Immunoprecipitation: ApreviouslotofthisantibodywasusedinIP. VisualizationofcellularandsubcellularlocalizationofS-100proteinusingimmunohistochemicaltechniques. Immunohistochemistry: A1:100-200dilutionofapreviouslotwasusedforparaffinembeddedtissuesections. Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	PurifiedbovineS-100protein
Epitope	Recognizesbothalpha&betaS100
Clone	15E2E2
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	Recognizesintact,alphaandbetasubunitsofbovine,human,andmurineS-100protein,asdeterminedbyimmunoprecipitationprofiles.
Isotype	IgG2aκ
SpeciesReactivity	Human Bovine Mouse Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_006271.1
EntrezGeneSummary	TheproteinencodedbythisgeneisamemberoftheS100familyofproteinscontaining2EF-handcalcium-bindingmotifs.S100proteinsarelocalizedinthecytoplasmand/ornucleusofawiderangeofcells,andinvolvedintheregulationofanumberofcellularprocessessuchascellcycleprogressionanddifferentiation.S100genesincludeatleast13memberswhicharelocatedasaclusteronchromosome1q21.ThisproteinmayfunctioninstimulationofCa2+-inducedCa2+release,inhibitionofmicrotubuleassembly,andinhibitionofproteinkinaseC-mediatedphosphorylation.Reducedexpressionofthisproteinhasbeenimplicatedincardiomyopathies.
GeneSymbol	S100A1 S100A S100 S100-alpha
PurificationMethod	ProteinAPurfied
UniProtNumber	P23297
UniProtSummary	FUNCTION:SwissProt:P23297#Weaklybindscalciumbutbindszincverytightly-distinctbindingsiteswithdifferentaffinitiesexistforbothionsoneachmonomer.Physiologicalconcentrationsofpotassiumionantagonizethebindingofbothdivalentcations,especiallyaffectinghigh-affinitycalcium-bindingsites. SIZE:94aminoacids;10546Da SUBUNIT:Dimerofeithertwoalphachains,ortwobetachains,oronealphaandonebetachain. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Highlyprevalentinheart.Alsofoundinlesserquantitiesinskeletalmuscleandbrain. SIMILARITY:SwissProt:P23297##BelongstotheS-100family.&Contains2EF-handdomains.

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Millipore/MAB079-1 | Anti-S-100 Protein Antibody, clone 15E2E2/MAB079-1/100 µL@ 1 mg/mL concentation

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