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            當前位置 : Millipore >>> Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL
            Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL
            • Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL

            Millipore/MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)/MAB1637/100 µL

            價格: ¥4308.00 市場價: 7180.00

            貨號: MAB1637
            品牌: Millipore
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              • Description
                CatalogueNumberMAB1637
                Replaces04-1049
                BrandFamilyChemicon®
                TradeName
                • Chemicon
                DescriptionAnti-TubulinAntibody,betaIIIisoform,CT,cloneTU-20(SimilartoTUJ1)
                AlternateNames
                • Tubulinbeta-III
                • tubulin,beta3
                • tubulin,beta4
                BackgroundInformationTubulinisoneofseveralmembersofasmallfamilyofglobularproteins.Themostcommonmembersofthetubulinfamilyareα-tubulinandβ-tubulin,theproteinswhichmakesupmicrotubules.Eachhasamolecularweightofapproximately55kDa.Microtubulesareassembledfromdimersofα-andβ-tubulin.Thesesubunitsareslightlyacidicwithanisoelectricpointbetween5.2and5.8.Tubulinwaslongthoughttobespecifictoeukaryotes.Recently,however,theprokaryoticcelldivisionproteinFtsZwasshowntobeevolutionarilyrelatedtotubulin.
                ProductInformation
                FormatAscites
                Control
                • Positivecontrol:Braintissueorneuronsofanytype.
                  Negativecontrol:NonneuraltissueorAstrocytes.
                PresentationMousemonoclonalIgG1ascitesinbuffercontainingPBScontaining1%BSAand15mMsodiumazide.
                StorageandShippingInformation
                StorageConditionsStablefor1yearat-20°Cinundilutedaliquotsfromdateofreceipt.
                HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
                Applications
                ApplicationAnti-TubulinAntibody,betaIIIisoform,C-terminus,cloneTU-20(SimilartoTUJ1)isanantibodyagainstTubulinforuseinELISA,IC,IH,IH(P),IP&WBwithmorethan55productcitations.
                KeyApplications
                • ELISA
                • Immunocytochemistry
                • Immunohistochemistry
                • Immunohistochemistry(Paraffin)
                • Immunoprecipitation
                • WesternBlotting
                ApplicationNotesImmunohistochemistry:
                Onformalinfixedfrozenorparaffinembeddedtissuesections:1:20to1:100dilutionfromapreviouslot.Hightemperaturecitratebufferantigenretrievalisrecommendedwithparaffinembeddedtissuesections.

                Immunoprecipitation:
                ApreviouslotofthisantibodywasusedonImmunoprecipitation.

                Immunocytochemistry:
                1:100to1:500dilutionfromapreviouslotwasusedonfixedcells.

                Immunoblotting:
                1:1000.Antibodywilldetectasinglebandat50kDacorrespondingtoclassIIIbeta-tubulin.

                ELISA:
                ApreviouslotofthisantibodywasusedonELISA.

                Optimalworkingdilutionmustbedeterminedbytheenduser.
                BIOLOGicalInformation
                ImmunogenAsyntheticpeptidecorrespondingtoaminoacids443-450(ESEAQGPK)ofhumanclassIIIbeta-tubulinconjugatedtoKLH.
                EpitopeC-terminus
                CloneTU-20(SimilartoTUJ1)
                ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
                HostMouse
                SpecificityTheantibodyreactswiththeC-terminusoftheneuronspecificbetaIIIisoformoftubulin.Doesnotidentifybetatubulinfoundinglialcells.
                IsotypeIgG1
                SpeciesReactivity
                • Avian
                • Bovine
                • Human
                • Mouse
                • Monkey
                • Pig
                • Rat
                • Sheep
                SpeciesReactivityNoteMouse.Expectedtocross-reactwithAvian,Porcine(Pig),Rat,Bovine,Sheep,Human,Monkey.Reactivitywithotherspecieshasnotbeenconfirmed.
                AntibodyTypeMonoclonalAntibody
                EntrezGeneNumber
                GeneSymbol
                • TUBB3
                • TUBB4
                • beta-4
                • MC1R
                PurificationMethodUnpurified
                UniProtNumber
                UniProtSummaryFUNCTION:SwissProt:Q13509#Tubulinisthemajorconstituentofmicrotubules.ItbindstwomolesofGTP,oneatanexchangeablesiteonthebetachainandoneatanon-exchangeablesiteonthealpha-chain.
                SIZE:450aminoacids;50433Da
                SUBUNIT:Dimerofalphaandbetachains.
                DOMAIN:SwissProt:Q13509ThehighlyacidicC-terminalregionmaybindcationssuchascalcium.
                SIMILARITY:Belongstothetubulinfamily.
                MolecularWeight55kDa
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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