| Immunogen | RatLcellstransfectedwiththecompletecodingsequenceofmouseFLK-1CDNA. | 
| Clone | 89B3A5 | 
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. | 
| Host | Rat | 
| Specificity | FLK-1isareceptortyrosinekinaseexpressedonendothelialcellsandonthecommonprecursorofendothelialandhematopoieticstemcellsAntibodiesagainstFLK-1aresuitableforstudiesforendothelialdevelopment,forembryonalhematopoiesis,andforsignaltransductionviatheFLK-1receptor.IthasnotbeentestedwhethertheantibodyblocksthebindingofVEGF. | 
| Isotype | IgG2a | 
| SpeciesReactivity |  | 
| AntibodyType | MonoclonalAntibody | 
| EntrezGeneSummary | ThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeABIlity,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms. | 
| GeneSymbol |  | 
| UniProtNumber |  | 
| UniProtSummary | FUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145. SIZE:232aminoacids;27042Da SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity). SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin. TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon. SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily. |