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Millipore/MAB2013 | Anti-K-Cadherin Antibody, extracellular domain, clone 2B6/MAB2013/100 µg

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貨號: MAB2013

品牌: Millipore

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Description
CatalogueNumber	MAB2013
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-K-CadherinAntibody,extracellulardomain,clone2B6
AlternateNames	Cadherin-6
BackgroundInformation	CadherinsconstituteafamilyoftransmembraneglycoproteinsinvolvedinCa2+-dependentcell-cellinteractions.Themembersofthisfamilyaredifferentiallyexpressedinvarioustissues.Theyfunctioninthemaintenanceoftissueintegrityandmorphogenesis.Thecadherinsgenerallycontainfiveextracellularrepeats,atransmembranedomainandacytoplasmictailthatbindstothecateninfamilyofcytoskeletalanchoringproteinswhichalsofunctionassignaltransducers.TheextracellulardomainsareresponsIBLeforthespecificityofhomophilicinteractionsbetweencellsexpressingthesamecadherin.CadherinsaredividedintotypeIandtypeIIsubgroups.TypeIcadherinsincludeepithelialcadherin(E-cadherin,cadherin-1oruvomorulin),neuralcadherin(N-cadherinorcadherin-2),placentalcadherin(P-cadherinorcadherin-3)andretinalcadherin(R-cadherinorcadherin-4).Kidneycadherin(K-cadherinorcadherin-6)andosteoblastcadherin(OB-cadherinorcadherin-11)aretypeIIcadherins.Cadherin-6isexpressedintheproximaltubuleepitheliumofthekidneyfromwhichmostrenalcellcarcinomasarederived.

ProductInformation
Format	Purified
Presentation	Purifiedimmuoglobulin.LiquidinPBScontaining0.09%sodiumazide.

StorageandShippingInformation
StorageConditions	1yearat4°Cfromdateofshipment

Applications
Application	Anti-K-CadherinAntibody,extracellulardomain,clone2B6detectslevelofK-Cadherin&hasbeenpublished&validatedforuseinWB,IC,IH.
KeyApplications	WesternBlotting Immunocytochemistry Immunohistochemistry
ApplicationNotes	Westernblot:1:25-1:250 Immunohistochemistry:1:25-1:50;Frozentissuesectionswithavidin-biotinylatedhorserADIshperoxidasecomplex(ABC)asthedetectionmethod. Optimalworkingdilutionsmustbedeterminedbytheenduser.I/

BIOLOGicalInformation
Immunogen	AffinitypurifiedextracellulardomainofhumanK-cadherin
Epitope	extracellulardomain
Clone	2B6
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	ReactswiththeextracellulardomainofK-cadherin.
Isotype	IgG1
SpeciesReactivity	Human Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_004932.2
EntrezGeneSummary	ThisgeneencodesatypeIIclassicalcadherinfromthecadherinsuperfamily.Theencodedmembraneproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Cadherinsmediatecell-cellbindinginahomophilicmanner,contributingtothesortingofheterogeneouscelltypesandthemaintenanceoforderlystructuressuchasepithelium.Strongtranscriptionalexpressionofthisgenehasbeenobservedinhepatocellularandrenalcarcinomacelllines,suggestingapossibleroleinmetastasisandinvasion.
GeneSymbol	CDH6 K-cadherin KCAD
UniProtNumber	P55285
UniProtSummary	FUNCTION:SwissProt:P55285#Cadherinsarecalciumdependentcelladhesionproteins.Theypreferentiallyinteractwiththemselvesinahomophilicmannerinconnectingcells;cadherinsmaythuscontributetothesortingofheterogeneouscelltypes. SIZE:790aminoacids;88309Da SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Highlyexpressedinbrain,cerebellum,andkidney.Lung,pancreas,andgastricmucosashowaweakexpression.Alsoexpressedincertainliverandkidneycarcinomas. SIMILARITY:SwissProt:P55285##Contains5cadherindomains.

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Millipore/MAB2013 | Anti-K-Cadherin Antibody, extracellular domain, clone 2B6/MAB2013/100 µg

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