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            當前位置 : Millipore >>> Millipore/MAB302 | Anti-Glutamine Synthetase Antibody, clone GS-6/MAB302/100 µg
            Millipore/MAB302 | Anti-Glutamine Synthetase Antibody, clone GS-6/MAB302/100 µg
            • Millipore/MAB302 | Anti-Glutamine Synthetase Antibody, clone GS-6/MAB302/100 µg

            Millipore/MAB302 | Anti-Glutamine Synthetase Antibody, clone GS-6/MAB302/100 µg

            價格: ¥5796.00 市場價: 9660.00

            貨號: MAB302
            品牌: Millipore
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              • Description
                CatalogueNumberMAB302
                BrandFamilyChemicon®
                TradeName
                • Chemicon
                DescriptionAnti-GlutamineSynthetaseAntibody,cloneGS-6
                BackgroundInformationGlutamineSynthetasecatalyzestheconversionofammoniaandglutamatetoglutamine.Itisfoundinastrocytesasanoctamerofidentical45kDasubunits.ThefunctionofGlutamineSynthetaseisthedetoxificationofbrainammonia.Italsohasanimportantroleinthemetabolicregulationofneurotransmitterglutamate.BecauseofthemultiplefunctionsandimportanceofGlutamineSynthetaseincellularmetabolism,bothcatalyticactivitiesandsynthesisarehighlyregulated.TheactivityofGlutamineSynthetaseiscontrolledbyadenylylation.ItsactivityisdecreasedinthecerebralcortexofbrainsaffectedbyAlzheimer"sdisease,particularlyinthevicinityofsenileplaques.Itisalsodecreasedunderconditionsofglucosedeprivation.ThelevelofexpressionofGlutamineSynthetaseisincreasedduringischemiainvivoorhypoxiainculture.
                ProductInformation
                FormatPurified
                Control
                • Ratbraintissue,ratbraincytosolicfractionextract
                PresentationPurifiedimmunoglobulin.Liquidinbuffercontaining50%glycerol,BSAand0.09%sodiumazide.
                StorageandShippingInformation
                StorageConditionsMaintainantibodyat2–8°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze-thawcycles.
                Applications
                ApplicationDetectGlutamineSynthetaseusingthisAnti-GlutamineSynthetaseAntibody,cloneGS-6validatedforuseinELISA,IH,IH(P)&WBwithmorethan45productcitations.
                KeyApplications
                • ELISA
                • Immunohistochemistry
                • Immunohistochemistry(Paraffin)
                • WesternBlotting
                ApplicationNotesWesternblot:1:1,000-1:5,000

                Immunohistochemistryonratbrain

                ELISA

                Optimalworkingdilutionsmustbedeterminedbyenduser.
                BIOLOGicalInformation
                ImmunogenGlutaminesynthetasepurifiedfromsheepbrain.
                CloneGS-6
                ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
                HostMouse
                SpecificityReactsstronglywithasinglebandonWesternblotsofsheepbrainandratbrain.Glutaminesynthetase,anenzymethatcatalyzestheaminationofglutamicacidtoformglutamine,isfoundinmammalsasanoctamerofeightidentical45kDasubunits.GlutaminesynthetaseactivityhasbeenshowntobeausefulMarkerofastrocytesandanimportantdifferentiationfeatureinretina.Glutaminesynthetaseisalsoconsideredtobeakeyenzymeintherecyclingoftheneurotransmitterglutamate.
                IsotypeIgG2a
                SpeciesReactivity
                • Human
                • Mouse
                • Rat
                • Sheep
                AntibodyTypeMonoclonalAntibody
                EntrezGeneNumber
                EntrezGeneSummaryGlutamineisamainsourceofenergyandisinvolvedincellproliferation,inhibitionofapoptosis,andcellsignaling(Haberleetal.,2005[PubMed16267323]).Fetalglutaminerequirementsareveryhighanddependlargelyonactiveglutaminesynthesisandthereleaseofglutamineintothefetalcirculationbytheplacenta.Glutaminesynthetase(EC6.3.1.2),alsocalledglutamate-ammonialigase(GLUL),isexpressedthroughoutthebodyandplaysanimportantroleincontrollingbodypHandinremovingammoniafromthecirculation.TheenzymeclearsL-glutamate,themajorneurotransmitterinthecentralnervoussystem,fromneuronalsynapses(seereferencesinClancyetal.,1996[PubMed8975719]).[suppliedbyOMIM]
                GeneSymbol
                • GLUL
                • GLNS
                PurificationMethodProteinAPurfied
                UniProtNumber
                UniProtSummaryFUNCTION:SwissProt:P15104#CatalyzestheconversionofammoniaandL-glutamatetoL-glutamine.
                SIZE:373aminoacids;42064Da
                SUBUNIT:Homooctamer.
                SUBCELLULARLOCATION:Cytoplasm.
                DISEASE:SwissProt:P15104#DefectsinGLULarethecauseofcongenitalsystemicglutaminedeficiency(CSGD)[MIM:610015].CSGDisararedevelopmentaldisorderwithseverebrainmalformationresultinginmulti-organfailureandneonataldeath.Glutamineislargelyabsentfromaffectedpatientsserum,urineandcerebrospinalfluid.
                SIMILARITY:SwissProt:P15104##Belongstotheglutaminesynthetasefamily.
                MolecularWeight45kDa
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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