| Immunogen | T-47Dbreastcarcinomaline. |
| Clone | 67A4 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | AntibodiesagainstE-cadherinaresuitabletocharacterizeepithelialcells.E-Cadherinisinvolvedinadhesionofepithelialcells(mostlyhomotypicandhomophilic).E-cadherinisalsoexpressedonimmatureerythroidcells.Theantibody67A4issuitabletostudyEcadherinexpressionbyflowcytometry.Inaddition,itblockstheinteractionofE-cadherinmoleculesandthereforeissuitabletostudyE-cadherin-mediatedsignaltransduction. |
| Isotype | IgG1 |
| SpeciesReactivity | |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneisaclassicalcadherinfromthecadherinsuperfamily.Theencodedproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Mutationsinthisgenearecorrelatedwithgastric,breast,colorectal,thyroidandovariancancer.Lossoffunctionisthoughttocontributetoprogressionincancerbyincreasingproliferation,invasion,and/ormetastasis.Theectodomainofthisproteinmediatesbacterialadhesiontomammaliancellsandthecytoplasmicdomainisrequiredforinternalization.Identifiedtranscriptvariantsarisefrommutationatconsensussplicesites. |
| GeneSymbol | - CDH1
- CDHE
- E-cadherin
- Arc-1
- Uvomorulin
- UVO
- Cadherin-1
- CD324
- uvomorulin
- ECAD
- LCAM
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P12830#E-Cad/CTF2promotesnon-amyloidogenicdegradationofAbetaprecursors.HasastronginhibitoryeffectonAPPC99andC83production.
SIZE:882aminoacids;97456Da
SUBUNIT:Homodimer;disulfide-linked.Interactsdirectly,viathecytoplasmicdomain,withCTNNB1orJUPtoformthePSEN1/cadherin/cateninadhesioncomplexwhichconnectstotheactinskeletonthroughtheactinbindingofalpha-catenin.InteractionwithPSEN1,cleavesCDH1resultinginthedisassociationofcadherin-basedadherensjunctions(CAJs).InteractswithAJAP1,CTNND1andDLG7.
SUBCELLULARLOCATION:Celljunction.Cellmembrane;Single-passtypeImembraneprotein.Note=ColocalizeswithDLG7atsitesofcell-cellcontactinintestinalepithelialcells.AnchoredtoactinMICROFILamentsthroughassociationwithalpha-,beta-andgamma-catenin.Sequentialproteolysisinducedbyapoptosisorcalciuminflux,resultsintranslocationfromsitesofcell-cellcontacttothecytoplasm.
TISSUESPECIFICITY:Non-neuralepithelialtissues.
PTM:Duringapoptosisorwithcalciuminflux,cleavedbyamembrane-boundmetalloproteinase(ADAM10),PS1/gamma-secretaseandcaspase-3toproducefragmentsofabout38kDa(E-CAD/CTF1),33kDa(E-CAD/CTF2)and29kDa(E-CAD/CTF3),respectively.Processingbythemetalloproteinase,inducedbycalciuminflux,causesdisruptionofcell-celladhesionandthesubsequentreleaseofbeta-cateninintothecytoplasm.Theresidualmembrane-tetheredcleavageproductisrapidlydegradedviaanintracellularproteolyticpathway.Cleavagebycaspase-3releasesthecytoplasmictailresultingindisintegrationoftheactinmicrofilamentsystem.Thegamma-secretase-mediatedcleavagepromotesdisaaaemblyofadherensjunctions.
DISEASE:"SwissProt:P12830#DefectsinCDH1areinvolvedindysfunctionofthecell-celladhesionsystem,triggeringcancerinvasion(gastric,breast,ovary,endometriumandthyroid)andmetastasis.&DefectsinCDH1areacauseofhereditarydiffusegastriccancer(HDGC)[MIM:137215].&DefectsinCDH1areacauseofsusceptibilitytoendometrialcancer[MIM:608089]."
SIMILARITY:SwissProt:P12830##Contains5cadherindomains. |
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