| Application | ThisAnti-OligodendrocytesAntibody,cloneCE-1isvalidatedforuseinIP,IC,IH,IH(P)forthedetectionofOligodendrocytes. | 
| KeyApplications | - Immunoprecipitation
 - Immunocytochemistry
 - Immunohistochemistry
 - Immunohistochemistry(Paraffin)
 
  | 
| ApplicationsNotRecommended |  | 
| ApplicationNotes | Immunocytochemistry
  Immunohistochemistryat1:1,000-1:5000
  NotsuggestedforuseinWesternblot.
  Optimalworkingdilutionsmustbedeterminedbyenduser.
  IMMUNOHISTOCHEMISTRYPROTOCOLFORMAB328
  Thisantibodyhasbeenusedsuccessfullyon30mm,freefloating,4%paraformaldehydefixedratbraintissue.Allstepsareperformedunderconstantagitation.Suggestedprotocolfollows.
  1)3x10minutewashesinTBS(withorwithout0.25%Triton).
  2)Incubatefor30minutesinTBSwith3%serum(sameashostfromsecondaryantibody).
  3)IncubateprimaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody)(withorwithout0.25%Triton)for2hoursatroomtemperaturefollowedby16hoursat4°C.
  4)3x10minutewashesinTBS.
  5)IncubatewithsecondaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody).
  6)3x10minutewashesinTBS.
  7)ABCElite(1:200VectorLabs)inTBS.
  8)2x10minutewashesinTBS.
  9)1x10minutewashinphosphatebuffer(nosaline).
  10)DABreactionwith0.06%NiCladdedforintensification.
  11)2x10minutewashesinPBS.
  12)1x10minutewashinphosphatebuffer(nosaline). |