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Millipore/MAB369 | Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt/MAB369/100 µL

價格: ￥3948.00 市場價: 6580.00

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貨號: MAB369

品牌: Millipore

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Description
CatalogueNumber	MAB369
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-DopamineTransporterAntibody,NT,cloneDAT-Nt
AlternateNames	DAT
BackgroundInformation	Thetransmembranedopaminetransporter(DAT)islocatedonthepresynapticnerveterminalandisresponsIBLeforterminatingdopaminergictransmissionbytransportingdopaminefromthesynapticcleftintothedopaminergicneuron(reuptake).Dopaminergicpathwayshavebeenstronglyimplicatedinrewardandaddiction,motivation,alcoholism,ADHDanddegenerativemotordiseasessuchasParkinson"s,Huntington"sandChorea.

ProductInformation
Format	CultureSupernatant
Control	PositiveControl:Brain(caudate,putamen,andnucleusaccumbens)tissue
Presentation	UnPurifiedtissueculturesupernatant,filteredthrougha0.2μmmembranepriortovialing.Productcontains20%FBSandCiprofloxacinatfinalconcentrationof10μg/mL.

StorageandShippingInformation
StorageConditions	Maintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

Applications
Application	ThisAnti-DopamineTransporterAntibody,N-terminus,cloneDAT-NtisvalidatedforuseinIC,IH,WBforthedetectionofDopamineTransporter.
KeyApplications	Immunocytochemistry Immunohistochemistry WesternBlotting
ApplicationNotes	Westernblot:Recognizesadiffusebandatapproximately70-85kDaonwesternblotsofextracts(20ugtotalprotein)fromhumancaudate,putamen,andnucleusaccumbens. Immunohistochemistry:4%paraformaldehydefixedtissue(careshouldbetakennottoover-fixtissue);perfusionfollowedbylessthan90minutespost-fixation,thencryoprotect.Suggestedworkingdilution1:1,000-1:10,000.Ifusingonrattissue,absorbedanti-ratsecondaryantibodiesarerecommended,andtheuseofratPAPsystems,orABCdetectionwillenhancesensitivity. Immunocytochemistry:1:5,000to1:10,000. Immunoblotting(notrecommendedforuseonrat) Optimalworkingdilutionsandprotocolsmustbedeterminedbyenduser. IMMUNOHISTOCHEMISTRYPROTOCOLFORMAB369 Thisantibodyhasbeenusedsuccessfullyon30μm,freefloating,4%paraformaldehydefixedmousebraintissue.Allstepsareperformedunderconstantagitation.Suggestedprotocolfollows. 1)3x10minutewashesinTBS(withorwithout0.25%Triton). 2)Incubatefor30minutesinTBSwith3%serum(sameashostfromsecondaryantibody). 3)IncubateprimaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody)(withorwithout0.25%Triton)for2hoursatroomtemperaturefollowedby16hoursat4°C. 4)3x10minutewashesinTBS. 5)IncubatewithsecondaryantibodydilutedappropriatelyinTBSwith1%serum(sameashostfromsecondaryantibody). 6)3x10minutewashesinTBS. 7)ABCElite(1:200VectorLabs)inTBS. 8)2x10minutewashesinTBS. 9)1x10minutewashinphosphatebuffer(nosaline). 10)DABreactionwith0.06%NiCladdedforintensification. 11)2x10minutewashesinPBS. 12)1x10minutewashinphosphatebuffer(nosaline).

BIOLOGicalInformation
Immunogen	N-terminusofhumandopaminetransporterfusedtoGlutathioneS-transferase.
Epitope	N-terminus
Clone	DAT-Nt
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rat
Specificity	RecognizesDopaminetransporter,N-terminus.ShowsnocrossreactivitytothecloselyrelatedSEROtoninandnorepinephrinetransporters(Miller,1997).ImmunolocalizationofDATonparaformaldehydefixedfrozensectionsofhumanbrainusingMAB369showsdensepunctatestainingthroughoutthecaudate,putamenandaccumbens(Miller,1997).
Isotype	IgG2aκ
SpeciesReactivity	Human Mouse Monkey Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_001044.3
EntrezGeneSummary	Thedopaminetransporter(DAT1)mediatestheactivereuptakeofdopaminefromthesynapseandisaprincipalregulatorofdopaminergicneurotransmission.TheDAT1genehasbeenimplicatedinhumandisorderssuchasparkinsonism,Tourettesyndrome,andsubstanceabuse(Vandenberghetal.,1992[PubMed1359373]).[suppliedbyOMIM]
GeneSymbol	SLC6A3 DAT1 ADHD ADD DAT
PurificationMethod	Unpurified
UniProtNumber	Q01959
UniProtSummary	FUNCTION:SwissProt:Q01959#Aminetransporter.Terminatestheactionofdopaminebyitshighaffinitysodium-dependentreuptakeintopresynapticterminals. SIZE:620aminoacids;68495Da SUBUNIT:Homooligomer;disulfide-linked.InteractswithPRKCABPandTGFB1I1. SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein. SIMILARITY:SwissProt:Q01959##Belongstothesodium:neurotransmittersymporter(SNF)family. MISCELLANEOUS:Thisproteinisthetargetofpsychomotorstimulantssuchasamphetaminesorcocaine.
MolecularWeight	~70-85kDa

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Millipore/MAB369 | Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt/MAB369/100 µL

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