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            當前位置 : Millipore >>> Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL
            Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL
            • Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL

            Millipore/MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9/MAB3757/100 µL

            價格: ¥3576.00 市場價: 5960.00

            貨號: MAB3757
            品牌: Millipore
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              • Description
                CatalogueNumberMAB3757
                BrandFamilyChemicon®
                TradeName
                • Chemicon
                DescriptionAnti-VEGFReceptor-3Antibody,extracellulardomain,clone9D9F9
                AlternateNames
                • VEGFR3
                • VEGFR-3
                • ReceptortyrosineKinaseVEGFR-3
                BackgroundInformationVascularendothelialgrowthfactorreceptor-3(VEGFR-3)anditsligands,vascularendothelialgrowthfactor-C(VEGF-C)and-D(VEGF-D),arethemajormoleculesinvolvedindevelopmentalandpathologicallymphangiogenesis.{Bando,2004}.JussilaetalreportedthatVEGFR3antibodiesspecificallystainendothelialcellsoflymphaticvesselsandvesselsaroundtumonrssuchaslymphomaandinsitubreastcarcinoma.{Jussila,1998}.Interestingly,thespindlecellsofseveralcutaneousnodularAIDS-associatedKaposi"ssarcomasandtheendotheliumaroundthenoduleswerealsoVEGFR-3positive.ThefirstspecificmolecularMarkerforthelymphaticendotheliumshouldprovideausefultoolfortheanalysisoflymphaticvesselsinmalignanttumorsandtheirmetastasesandthecellularoriginanddifferentiationofKaposi"ssarcomas.{Jussila,1998}.
                ProductInformation
                FormatAscites
                Control
                • Humanlymphatictissues
                • VEGFR-3isnotexpressedinHEVsofthelymphnodes.{Jussila,1998].Inlymphomasstainingisseenmostlyincollapsedvesselsinthecortexoflymphnodesinfiltratedbythelymphomacells.Theantibodyspecificallydetectslymphaticendotheliuminhumanlymphnodeandotherorganssuchastonsil.SeeJussilaetal,1998forcompletetissuecharacterization.
                PresentationUnPurifiedascitesfluidcontaining0.1%sodiumazideasapreservative.
                StorageandShippingInformation
                StorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
                Applications
                ApplicationAnti-VEGFReceptor-3Antibody,extracellulardomain,clone9D9F9isanantibodyagainstVEGFReceptor-3foruseinFC,IF,IH(P),IP&WB.
                KeyApplications
                • FlowCytometry
                • Immunofluorescence
                • Immunohistochemistry(Paraffin)
                • Immunoprecipitation
                • WesternBlotting
                ApplicationNotesImmunohistochemistry:frozencryosections,5μmcryosectionswereair-driedandfixedincoldacetonefor10minutes.Blockedwith5%normalhorseserumandthenincubatedwith9D9for2hoursatroomtemperatureinahumidchamber.Typicaldilutionswillrangefrom1:100-1:1000dependinguponlottiter.Detectioniswithenhancedmethods(ABCorpolyHRP)andenzymaticsubstratesforbestresults.

                Immunohistochemistryinparaffin:5μmthicklightlyfixedformalintissueswereprocessedthroughdecreasingethanolconcentrations,washedwithwater,thenheatedinamicrowavein10mMsodiumcitratepH6.0at780Wfor5minutes,followedby450Wfor10minutes.Thesectionswerethenincubatedinmethanolcontaining30%hydrogenperoxidefor30minutesandprocessedfurtherlikethecryosections.{Jussila,1998}.

                WesternBlot:antibodyrecognizesVEGFR-3onreducedsamplesofHUVECandHDMECcells{primayhumanumbilicalveinendothelialcells&primaryhumandermalmicrovascularendothelialcells}.Thebandsseenatmolecularweight195kDaand125kDarepresenttheuncleavedandproteolyticallycleavedformsofVEGFR3.Thebandat175kDaisconsideredtobeintracellular,unglycosylatedprecursor{Bando,2004}.

                Immunoprecipitation:1-5μLper300-500μLofcelllysate.UseproteinAorrabbitanti-mouseIgGcaptureforbestresults.

                ELISA:9D9F9hasbeenusedasthedetectionantibodyforaVEGFR-3ELISA{Bando,2004}.

                FlowCytometry:reactiveontransfectedNIH-3T3cellsexpressinghVEGFR3,Othercelllinesuntested.{Jussila,1998}.

                Optimaldilutionswillneedtobedeterminedbytheenduser.
                BIOLOGicalInformation
                ImmunogenRecombinantlyproduced,purified,hVEGFR-3extracellulardomainproteinfrombaculovirusculture{Jussila,1998}.
                Epitopeextracellulardomain
                Clone9D9F9
                ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
                HostMouse
                SpecificitySpecificforhumanVEGFR-3.WesternblotandELISAdemonstratethattheantibodyishighlyspecificforVEGFR-3withnocross-reactivitywithhumanVEGFR-1orVEGFR-2protein{Bando,2004}.CrossreactivitywithotherspeciesVEGFR-3proteinsuntested.
                IsotypeIgG1
                SpeciesReactivity
                • Human
                AntibodyTypeMonoclonalAntibody
                EntrezGeneNumber
                GeneSymbol
                • VEGFR3
                PurificationMethodUnpurified
                UniProtNumber
                MolecularWeight175kDa
                PhysicochemicalInformation
                Dimensions
                MaterialsInformation
                MaterialsInformation
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