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Millipore/MAB5272 | Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324/MAB5272/100 µg

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貨號: MAB5272

品牌: Millipore

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Description
CatalogueNumber	MAB5272
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-NeuralCellAdhesionMoleculeL1Antibody,clone324
AlternateNames	CD171 N-CAML1
BackgroundInformation	Familiesofadhesionmoleculeswhichsharecommoncarbohydratedomainsdoexist,despitethestructuralandfunctionaldiversityoftheseglycoproteins.TheseincludetheCa2+-independentneuraladhesionmolecules:N-CAM,myelinassociatedglycoprotein(MAG)andL1.L1isinvolvedinneuron-neuronadhesion,neuritefasciculation,outgrowthofneurites,cerebellargranulecellmigration,neuriteoutgrowthonSchwanncellsandinteractionsamongepithelialcellsofintestinalcrypts

ProductInformation
Format	Purified
Control	Braintissue
Presentation	Ratmonoclonalinbuffercontaining0.02MPhosphatebuffer,0.25MNaCl,pH7.6with0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8oCfromdateofreceipt.

Applications
Application	ThisAnti-NeuralCellAdhesionMoleculeL1Antibody,clone324isvalidatedforuseinIC,IH,WBforthedetectionofNeuralCellAdhesionMoleculeL1.
KeyApplications	Immunocytochemistry Immunohistochemistry WesternBlotting
ApplicationNotes	Westernblotting: 20μg/mLwasusedonapreviouslot. Immunocytochemistry: 5-10μg/mLwasusedonapreviouslot.Cellsmustbefixedwithmethanolfor10minutesat-20°Cinthemiddlelogphase. Immunohistochemistry:Clone324issensitivetofixation.Freshfrozen,acetoneor-20Cmethanolfixedtissuesarerecommended.TrADItional4%PFAtypicallydoesnotworkwell.Otherfixativeshavenotbeentested. Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	Ratswereimmunizedwithaglycoproteinfractionfromcerebellumof8-10dayoldC57BL/6Jmice.
Clone	324
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rat
Specificity	MonoclonalantibodyagainstneuralcelladhesionmoleculeL1fromrat-rathybridcells.
Isotype	IgG
SpeciesReactivity	Mouse Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_032504.3
EntrezGeneSummary	ThisgeneisamemberoftheIgsuperfamilyandencodesacellsurfacesialoglycoproteinexpressedbycytokine-activatedendothelium.ThistypeImembraneproteinmediatesleukocyte-endothelialcelladhesionandsignaltransduction,andmayplayaroleinthedevelopmentofartherosclerosisandrheumatoidarthritis.Twoalternativelysplicedtranscriptsencodingdifferentisoformshavebeendescribedforthisgene.
GeneSymbol	VCAM1 INCAM-100 MGC99561 CD106 DKFZp779G2333 L1CAM
PurificationMethod	ProteinAPurfied
UniProtNumber	P11627
UniProtSummary	FUNCTION:SwissProt:P19320#Importantincell-cellrecognition.Appearstofunctioninleukocyte-endothelialcelladhesion.Interactswiththebeta-1integrinVLA4onleukocytes,andmediatesbothadhesionandsignaltransduction.TheVCAM1/VLA4interactionmayplayapathophysiologicrolebothinimmuneresponsesandinleukocyteemigrationtositesofinflammation. SIZE:739aminoacids;81276Da SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Expressedoninflamedvascularendothelium,aswellasonmacrophage-likeanddendriticcelltypesinbothnormalandinflamedtissue. DOMAIN:SwissProt:P19320EitherthefirstorthefourthIg-likeC2-typedomainisrequiredforVLA4-dependentcelladhesion. PTM:Sialoglycoprotein. DISEASE:SwissProt:P19320#Mayplayanimportantroleinthegenesisofatherosclerosisandrheumatoidarthritis. SIMILARITY:Contains7Ig-likeC2-type(immunoglobulin-like)domains.
MolecularWeight	120-140kDa

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Millipore/MAB5272 | Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324/MAB5272/100 µg

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