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Millipore/MAB5292 | Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B/MAB5292/100 µg

價格: ￥3948.00 市場價: 6580.00

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貨號: MAB5292

品牌: Millipore

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Description
CatalogueNumber	MAB5292
Replaces	AB1791P
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-DopamineD1BReceptorAntibody,cloneSG4-D1B
AlternateNames	DopamineD5Receptor

ProductInformation
Format	Purified
Presentation	Purifiedimmunoglobulin.Liquidin0.02MPhosphatebuffer,0.25MNaCl,pH7.6with0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Maintainat2-8°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.

Applications
Application	ThisAnti-DopamineD1BReceptorAntibody,cloneSG4-D1BisvalidatedforuseinIH,WBforthedetectionofDopamineD1BReceptor.
KeyApplications	Immunohistochemistry WesternBlotting
ApplicationNotes	Westernblot:1:250-1:500onmousebrainlysate(unboiledsample) Immunohistochemistry Optimalworkingdilutionsmustbedeterminedbytheenduser. SUGGESTEDWESTERNBLOTPROTOCOL Solutions 10XTransferbuffer1liter Tris115.3gm Glycine24.2gm [SDS4gm(maybeomitted)] Fora1Xworkingsolution:1liter mix700mLwater 200mL MeOH100mL 10XTransfer TBSTstock1liter1M TrispH7.510mL 5MNaCl30mL 10%Tween-205Ml 1.RunSDS-PAGEgelasdesired. 2.SoakPVDFmembrane(ImmobilonPfromMillipore)in100%methanolfor1-2minutestowetthemembrane. 3.Soakmembraneintransferbuffer(seebelow)untilreadytosetupblot(>15min)transfer. 4.Assemblegel-transfersandwichwiththeblottowardthepositiveelectrode. 5.Transferproteinfromgeltomembraneat100voltsfor1-2hoursforsmallmolecularweightproteinsor65mAfor16hours(overnight)forcompletetransferofhighermolecularweightproteins. 6.StainthetransferredbandswithChemiconBLOT-FastStain(CatalogNumber2076). 7.Destainwithdeionizedwater. 8.Blockwith5%non-fatmilk(MarvelorCarnation)inwater,overnightat4°C.Thenon-fatmilkshouldbedissolvedfreshly,centrifuged10,000rpmfor10min,andfilteredthroughglassfilter(GelmanAcrodisc). 9.Incubationwithfirstantibodyovernightwithrockingat4°Cinblockingsolution.Optimalworkingdilutionsandincubationtimewillneedtobedeterminedbytheenduser. 10.Washatleast3x5min.withTBST.Fromthisstage,azideshouldbeomitted. 11.Incubationwiththesecondaryantibody(HRP-conjugatedgoatanti-mouseantibody,forexampleChemiconCatalogNumberAP124P,dilutedappropriately)1hatroomtemperature. 12.Washatleast3x5min.withTBST. 13.PerformECLwithcommercialkit(femtoLUCENT,ChemiconCatalogNumber2078).

BIOLOGicalInformation
Immunogen	RecombinantratD1bReceptor,carboxyterminus.
Clone	SG4-D1B
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	DopamineReceptorD1b(D5).
Isotype	IgG1
SpeciesReactivity	Mouse Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_000798.3
EntrezGeneSummary	ThisgeneencodestheD5subtypeofthedopaminereceptor.TheD5subtypeisaG-proteincoupledreceptorwhichstimulatesadenylylcyclase.Thisreceptorisexpressedinneuronsinthelimbicregionsofthebrain.Ithasa10-foldhigheraffinityfordopaminethantheD1subtype.Pseudogenesrelatedtothisgeneresideonchromosomes1and2.
GeneSymbol	DRD5 DRD1B DRD1L2 DBDR D MGC10601
Non-ReactiveSpecies	Human
UniProtNumber	P21918
UniProtSummary	FUNCTION:SwissProt:P21918#Thisisoneofthefivetypes(D1toD5)ofreceptorsfordopamine.TheactivityofthisreceptorismediatedbyGproteinswhichactivateadenylylcyclase. SIZE:477aminoacids;52951Da SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein. TISSUESPECIFICITY:Neuron-specific,localizedprimarilywithinlimbicregionsofthebrain. DISEASE:SwissProt:P21918#DefectsinDRD5maybeacauseofschizophrenia,butnoproofhasyetbeenfound.&DefectsinDRD5areacauseofblepharospasm[MIM:606798].Itisaprimaryfocaldystoniaaffectingtheorbicularisoculimuscles,usuallybeginninginmiddleage.Initialsymptomsincludeeyeirritationandfrequentblinking,progressingtoinvoluntaryspasmsofeyelidclosure.Inseverecases,thiscanleadtofunctionalblindness. SIMILARITY:SwissProt:P21918##BelongstotheG-proteincoupledreceptor1family.

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Millipore/MAB5292 | Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B/MAB5292/100 µg

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