當前位置： Millipore >>> Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL

Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL

價格: ￥4308.00 市場價: 7180.00

打印產品信息

貨號: MAB5300

品牌: Millipore

規格

默認

數量

庫存(0)

特別提示

代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
下單后請及時聯系客服核對商品價格，訂單生效后再付款。

立即購買加入購物車

收藏商品

資深產品顧問

咨詢顧問

全國免費服務熱線

4000-520-616

自營商城一站式服務
廠家直采剔除溢價
品質甄選正品保證
嚴控流程只做188精品
極速物流如約送貨

詳情
使用說明
常見問題

Description
CatalogueNumber	MAB5300
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-DopamineAntibody,cloneK56A

ProductInformation
Format	Purified
Presentation	Purifiedimmunoglobulin.Liquidcontaining50%glycerol.

StorageandShippingInformation
StorageConditions	Maintainat-20°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.

Applications
Application	Anti-DopamineAntibody,cloneK56AdetectslevelofDopamine&hasbeenpublished&validatedforuseinIH.
KeyApplications	Immunohistochemistry
ApplicationNotes	Immunohistochemistry:1:500-1:2,500usingfreefloatingsectionsbythePAPtechniqueonratdopaminergicareas. Optimalworkingdilutionsmustbedeterminedbyenduser. PROTOCOLforDopamineDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain. 1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded. SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2. SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5. 2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min. 3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC). 4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused. 5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride. 6.APPLICATIONOFDOPAMINEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+4°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure. 6.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-rabbitinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionB. 7.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionBcontaining1%non-specificserumfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionB. 8.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.WashtissuewithsolutionDusing2,10minwashes.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia. Forresearchuseonly;notforuseasadiagnostic.

BIOLOGicalInformation
Immunogen	Dopamine-Gluteraldehyde-BSA.
Clone	K56A
Host	Mouse
Specificity	Dopamine. Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds: CompoundCross-reactivity Dopamine-G-BSA1 L-DOPA-G-BSA1/10,000 Tyrosine-G-BSA1/36,000 Tyramine-G-BSA1/>50,000 Noradrenaline-G-BSA1/>50,000 Octopamine-G-BSA1/>50,000 Adrenaline-G-BSA1/>50,000 Dopamine1/>50,000 Abbreviations: (G)Glutaraldehyde (BSA)BovineSerumAlbumin
Isotype	IgG
SpeciesReactivity	Rat
AntibodyType	MonoclonalAntibody

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

售后保障

螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。

Q：生物188產品正品保障嗎？
A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購，自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

Q：下單后可以修改訂單嗎？
A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

Q：可以開發票嗎？
A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

Q：商品幾天可以發貨？
A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

Q：如何聯系商家？
A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

Q：收到的商品少了/發錯了怎么辦？
A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

Q：退換貨/維修需要多長時間？
A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

24小時熱銷榜

Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
￥830.00
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
￥830.00
Qubit? dsDNA BR Assay Kit Q32853現貨
￥2000.00
Parafilm M封口膜/PM996/4x125/整箱
￥1296.00

Millipore/MAB5300 | Anti-Dopamine Antibody, clone K56A/MAB5300/100 µL

客戶服務

何為188

關注188微信公眾號