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Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

價格: ￥4308.00 市場價: 7180.00

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貨號: MAB5304

品牌: Millipore

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Description
CatalogueNumber	MAB5304
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-GlutamateAntibody

ProductInformation
Format	Purified
Presentation	LiquidinPBScontaining10mMsodiumazide.

StorageandShippingInformation
StorageConditions	Maintainat2-8°Cinundilutedaliquotsforupto6monthsafterdate.

Applications
Application	Anti-GlutamateAntibodydetectslevelofGlutamate&hasbeenpublished&validatedforuseinIH.
KeyApplications	Immunohistochemistry
ApplicationNotes	Immunohistochemistry:1:2,500-1:10,000usingfreefloatingsectionsbythePAPtechniqueonrathippocampus. Optimalworkingdilutionsmustbedeterminedbyenduser. PROTOCOLforGlutamateDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain. 1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded. SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2. SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5. 2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min. 3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC). 4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused. 5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride. 6.APPLICATIONOFGLUTAMATEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+2-8°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure. 7.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-mouseinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor1-2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionC. 8.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionCfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionC. 9.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia. Forresearchuseonly;notforuseasadiagnostic.

BIOLOGicalInformation
Immunogen	Glutamate-Glutaraldehyde-BSA.
Host	Mouse
Specificity	Glutamate Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds: CompoundCross-reactivity Glutamate-G-BSA1 Aspartate-G-BSA1/100,000 GABA-G-BSA1/100,000 Glutamate1/100,000 Abbreviations: (G)Glutaraldehyde (BSA)BovineSerumAlbumin NOTE:AntibodyreactivityREQUIRESglutaraldehydefixationthussomeglutaraldehyde(0.5%-2.0%)needstobeincludedinthetissuefixationprocedureinorderfortheproperreactivity.
Isotype	IgM
SpeciesReactivity	Rat
AntibodyType	MonoclonalAntibody

PhysicochemicalInformation

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Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

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