| Immunogen | Humanbonemarrowstromalcellline,SV40transformed. |
| Clone | 7.1 |
| Host | Mouse |
| Specificity | NG2(chondroitinsulfateproteoglycan),humanwhichisthehomologueoftheratNG2molecule.Theantibodyreactswitha220-240kDsurfaceproteinwhoseN-terminalsequenceisidenticaltotheratNG2chondrotinsulfateproteoglycanmolecule.
MAB5520reactswithonlyneoplasticleukocyteswithspecificassociationwithchromosome11q23translocationsinvolvingtheMLLgene.Ithasaveryhighspecificityfort(4;11)ort(11;19)translocations.Theantibodyshowsnoreactivitywithnormalmarrowhematopoieticcells,peripheralblldleukocytes,redbloodcellsorplatelets.LeukemiccellslinesKG1,KG1a,HRL,Jurkat,K562,Raji,HL-60,U937,Daudi,Nalm6andCEMarenegative.HoweverMAB5520doesreactwithavarietyofcelllinesofnonhematopoieticorigin,includingHeLacells. |
| Isotype | IgG1 |
| SpeciesReactivity | |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Ahumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreADIngonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells. |
| GeneSymbol | - CSPG4
- MSK16
- MCSP
- NG2
- MCSPG
- MEL-CSPG
- HMW-MAA
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades.
SIZE:2322aminoacids;250497Da
SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16.
SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity).
TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells.
PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity).
SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains.
MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells. |
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