Mouse monoclonal antibody raised against native Actin.
Immunogen:
Native purified Actin from Chicken gizzard.
Host:
Mouse
Theoretical MW (kDa):
42
Reactivity:
Chicken, Human, Yeast
Specificity:
This antibody reacts with all known actins from yeast to man. specific to the ~42 KDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms.
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
Western Blot (1:5000)Immunofluorescence (1:500)Immunohistochemistry (1:500)The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Publication Reference
1.
NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane.Kotak S, Busso C, Gonczy PEMBO J. 2014 Jul 4. pii: e201488147.
2.
Rab18 Facilitates Dengue Virus Infection by Targeting Fatty Acid Synthase to Sites of Viral Replication.Tang WC, Lin RJ, Liao CL, Lin YLJ Virol. 2014 Apr 2.
3.
NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function.Kotak S, Busso C, Gonczy PEMBO J. 2013 Sep 11;32(18):2517-29. doi: 10.1038/emboj.2013.172. Epub 2013 Aug 6.
4.
Cortical dynein is critical for proper spindle positioning in human cells.Kotak S, Busso C, Gonczy P.J Cell Biol. 2012 Oct 1;199(1):97-110. doi: 10.1083/jcb.201203166.
5.
Two monoclonal antibodies to actin: one muscle selective and one generally reactive.Lessard JL.Cell Motil Cytoskeleton. 1988;10(3):349-62.
Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein.
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