Synonym:MSQual/QuantQCMixProperties:
RelatedCategories | AminoAcid,Peptide&ProteinStandards,AnalyticalStandards,Analytical/Chromatography,MassSpectrometry,MolecularBIOLOGy, |
form | lyophilizedpowder |
shippedin | ambient |
storagetemp. | 2-8°C |
Description:
Components
Eachvialcontains25μgoflyophilizedpeptidesthatarederivedfromtrypsindigestionoftheproteinconcatamerQCAL.
Application
MSQC2isintendedtoactasauniversalMSplatformstandard,byprovidingseveralelementsforcalibrationandperformanceassessment,suchasinstrumentresolutionandlinearityofsignaldetection.
TheMSQual/QuantQCMixisaLC-MSstandarddesignedtobenchmarkandmonitordailyperformanceofbothqualitativeandquantitativeproteomicplatforms.MSQual/QuantQCMixisaninjectionreadystandard,designedwithmultiplereactionmonitoring/selectedreactionmonitoring(MRM/SRM)methodologyinmind.Thisproductisoptimizedtoassessplatformcharacteristics,including:
• Reproducibilitybetweenruns
• SystemstABIlity(drift,chromatography,signalintensity,sensitivity,etc.)
• Inter-andintra-platformandlabcomparisons
Generaldescription
QCALwasdesignedusingtheQconCATtechnologyandrecombinantlyexpressedinE.coli.?TheparentproteinsequenceofQCALisasfollows:
MGALRVFDEFKPLVEEPQNLIRVFDEFKPLVKPEEPQNLIRVFDEFKPLVKPEEKPQNLIRVFDEFKPLVKPEEKPQNKPLIRVFKPDEFKPLVKPEEKPQNKPLIRVFKPDEFKPLVKPEEKPQNKPLIKPRVFDEFQPLVEEPQNLIRGVNDNEEGFFSARGGVNDNEEGFFSARGGVNDNEEGFFSARGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGVNDNEEGFFSAKGGGVNDNEEGFFSARAVMDDFAAFVEKAVMMDDFAAFVEKAVMMMDDFAAFVEKGLVKFVVPRALELFRIGDYAGIKEALDFFARYLGYLEQLLRVLYPNDNFFEGKLFTFHADICTLPDTEKALVALVLVPRGSLEVLFQGPIEGRTENLYFQGDDDDKALVALVHHHHHH
QCALwassubsequentlydigestedwithtrypsintogiveacoremixtureof22peptides.FeaturesandBenefits
General
Complexity
• Definedmixturegivesconfidenceinyourinstrumentsanalysis
Dynamicrange
• Generatedatapointsthatsimulatetherangeofrealworldconditions
Predigested
• Eliminatevariability
Injectionready
• Decreasepreptime
Qualitative
Mixtureof6trypticallydigestedhumanproteins(FASTAfile)
• Lowcomplexity,definedmixture
• 25foldconcentrationrange
• C18purified
14SILpeptides
• SILpeptidesspanaconcentrationrangegreaterthanthreeordersofmagnitude(Table1)
• 2-3peptidesperprotein
Quantitative
• MRMsettingsprovided(Skyline,xls,csv)
• MRMtransitionsprovided(filesabove)
• Light:Heavyratiosfrom50:1to1:5
Safety:
RIDADR
NONHforallmodesoftransport