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            當前位置 : Millipore >>> Millipore/MT17MAG47K-PX25 | MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Premixed 25 plex - Immunology
            Millipore/MT17MAG47K-PX25 | MILLIPLEX <small>MAP</small> Mouse TH17 Magnetic Bead Panel - Premixed 25 plex - Immunology
            • Millipore/MT17MAG47K-PX25 | MILLIPLEX <small>MAP</small> Mouse TH17 Magnetic Bead Panel - Premixed 25 plex - Immunology

            Millipore/MT17MAG47K-PX25 | MILLIPLEX MAP Mouse TH17 Magnetic Bead Panel - Premixed 25 plex - Immunology

            價格: ¥46716.00 市場價: 77860.00

            貨號: MT17MAG47K-PX25
            品牌: Millipore
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              • Description
                CatalogueNumberMT17MAG47K-PX25
                TradeName
                • MILLIPLEX
                DescriptionMILLIPLEXMAPMouseTH17MagneticBeadPanel-Premixed25plex-ImmunologyMultiplexAssay
                OverviewTheMILLIPLEXMAPMouseTh17MagneticBeadpanelenablesyoutofocusonthetherapeuticpotentialofthemodulationofCD4T-helpercellcytokines.CoupledwiththeLuminex?xMAP?platforminamagneticbeadformat,youreceivetheadvantageofidealspeedandsensitivity,allowingquantitativemultiplexdetectionofdozensofanalytessimultaneously,whichcandramaticallyimproveproductivity.
                BackgroundInformationCD4T-helpercellsaremajorplayersinadaptiveimmunity.Basedontheirexpressionprofileoftranscriptionfactorsandsecretedcytokines,thesecellsaredividedintothemajorsubsetsTh1,Th2,Th17andTregulatory(Treg)cells.Th1cells,inducedbyIL-12andenhancedbyIFN?,secreteIFN?thatactivatesmacrophages.InducedbyIL-4,Th2cellsprimarilyproduceIL-4,IL-5,IL-13,IL-25andIL-17E,playingaroleinallergy.Tregcells,inducedbyTGFβ,producetheanti-inflammatorycytokinesIL-10andTGFβandfunctiontocontrolT-cellresponsestopreventautoimmunereactions.Th17cells,amorerecentlydiscoveredsubsetcharacterizedbytheproductionofIL-17,areinducedbyTGFβcombinedwithIL-23andIL-1βinhumans,andTGFβ,IL-6andIL-21inmice.ActivatedTh17cellssecreteIL-17A,IL-17F,IL-21,IL-22andTNFαtopromotetissueinflammation.

                Th17cellsareinvolvedintheclearanceofextracellularbacteriaandfungi.TheyareabundantintheintestinallaminapropriaandfunctionasabarrieragainstinvADIngpathogens.ExcessiveamountsofTh17cellshavebeenimplicatedinthepathogenesisofseveralautoimmunediseases,includingmultiplesclerosis,psoriasis,juvenilediabetes,rheumatoidarthritis,Crohn"sdiseaseandautoimmuneuveitis.InadditionTh17cellsplayanimportantroleintumordevelopment,progressionandmetastasis,potentiallyinbothpromotingandinhibitingtumorgrowth.
                ProductInformation
                DetectionmethodLuminexxMAP
                ConfigurationPremixed
                Precision,%
                • Inter-assay:<>
                • Intra-assay:<>
                PanelTypeMAGNETICCytokines/Chemokines
                StorageandShippingInformation
                StorageConditionsRecommendedstorageforkitcomponentsis2-8°C.
                Applications
                ApplicationSimultaneouslyanalyzemultipleTh17cytokineandchemokinebioMarkerswiththeTh17Bead-BasedMultiplexAssaysusingtheLuminextechnology,inhumanserum,plasmaandcellculturesamples.
                KeyApplications
                • Multiplexing
                ApplicationNotes?Thisisatwo-hourorovernightincubationassayrequiring25μLofneatplasmaorserum,or25μLcellculturesupernatantperwell.
                ?ThiskitmaybeusedfortheanalysisofalloranycombinationoftheTh17panelanalytesinserum,plasmaortissue/celllysateandculturesupernatant
                BIOLOGicalInformation
                SpecificityCross-reactivitybetweentheantibodiesandanyoftheotheranalytesinthispanelisnon-detectableornegligIBLe.
                SpeciesReactivity
                • Mouse
                AnalytesAvailable
                • CD40Ligand
                • GM-CSF
                • IFN-γ
                • IL-1β
                • IL-2
                • IL-4
                • IL-5
                • IL-6
                • IL-10
                • IL-12(p70)
                • IL-13
                • IL-15
                • IL-17A
                • IL-17E/IL-25
                • IL-17F
                • IL-21
                • IL-22
                • IL-23
                • IL-27
                • IL-28B
                • IL-31
                • IL-33
                • MIP-3α/CCL20
                • TNF-α
                • TNFβ
                CrossReactivityTherewasnoorminimumcross-reactivitybetweentheantibodiesandanyotheranalytesinthepanel(seedetailsinassayprotocol).
                Accuracy
                • 85-110%
                PhysicochemicalInformation
                Sensitivity
                • Refertokitprotocolforsensitivitiesofindividualbiomarkers.
                LinearityofDilution1:4111.0%1:8113.1%
                StandardCurveRange
                • CD40LIL-31&MIP-3α:49–50,000pg/mL
                • GM-CSF&IL-15:34–35,000pg/mL
                • IFNγ&IL-6:7.8–8,000pg/mL
                • IL-1β:15–15,000pg/mL
                • IL-2:6.9–6,000pg/mL
                • IL-4:1.5–1,500pg/mL
                • IL-5:4.9–5,000pg/mL
                • IL-10,IL-12(p70),&IL-21:20–20,000pg/mL
                • IL-13&IL-17A:39–40,000pg/mL
                • IL-17E/IL-25:586–600,000pg/mL
                • IL-17F:10–10,000pg/mL
                • IL-22:2.4–2,500pg/mL
                • IL-23:342-350,000pg/mL
                • IL-27:879–900,000pg/mL
                • IL-28B:127–130,000pg/mL
                • IL-33:78–80,000pg/mL
                • TNFβ:488–500,000pg/mL
                • TNFα:3.4–3,500pg/mL
                Dimensions
                MaterialsInformation
                MaterialsInformation
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