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Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

價格: ￥6288.00 市場價: 10480.00

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貨號: NS830

品牌: Millipore

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Description
CatalogueNumber	NS830
BrandFamily	Chemicon®
TradeName	Chemicon
Description	GFAPELISAKit
Overview	Background GlialFibrillaryAcidicProtein(GFAP)isaclass-IIIintermediatefilamentuniquelyandubiquitouslyexpressedaspartofthestructuralnetworkinastrocyticglialcells.ThisspecificexpressionmakesGFAPidealasabroadastrocyticMarkerinvertebrates.Astrocytesplayavarietyofkeyrolesinsupporting,guiding,nurturing,andsignalingneuronalarchitectureandactivityinahighlyinterdependentmanner.CurrentunderstandingofCNSstructuresuggeststhatthereareatleastasmanyglialcellsastheestimated100billionneurons,butperhapsupwardsof100timesmore.Thus, thehealthofbothneuronsandastrocytesisoftenaffectedindiseaseandtrauma.GFAPisprovingtobeausefulbiomarkerinthedetectionofneuraldegeneration.AlthoughdefectsinGFAPhavebeenreportedtocausecertainrareconditions,suchasAlexander’sdisease(Brenneretal.,2001),theobservedinterconnectedphysiologyofneuronalandglialcellshasenabledtheuseofGFAPasaneurodegenerationdetectiongauge.Thisrelationshipisbeingexploitedbyresearchersandcliniciansmeasuringavarietyofneurologicalissuesfromcancertotrauma.GFAPhasbeenusedasabiomarkerintheearlydetectionofchemicallyinducedcancer(Capoetal.,1997)andclassicalglioblastomas(Jungetal.,2007).ElevatedlevelsofGFAPhavebeenreportedintraumaticbraininjury(TBI)andappeartodirectlycorrelatewiththeseverityofinjuryandbraintissueoxygenationincombatcasualtycareresearch(CCR,2008)aswellascivilianaccidents(Mondelloetal.,2010).GFAPhasalsobeenidentifiedasagoodbiomarkerinstudiesoftoxininducedacutebraininjury(Liaoetal.,2008),indifferentiationofhemorrhagicvs.ischemicstrokes(Foerchetal.,2006),aswellasincardiacarrest(Kanekoetal.,2009).MilliporenowoffersanELISAassayforsensitivemeasurementofGFAPproteinintissuehomogenatesandBIOLOGicalfluids(serum,plasma,CSF,andserumfreemedia)fromhuman,mouse,andrat. PrincipalsofProcedure ThisassayisaSandwichELISAbasedsequentiallyon:1)captureofsolubleGFAPproteintothewellsofamicrotiterplatecoatedbyapre-titeredamountofanti-GFAPmonoclonalantibody;2)removalofunboundmaterialbywashing;3)bindingofbiotinylatedanti-GFAPmonoclonalantibodyto thepreviouslycapturedGFAPprotein;4)removalofunboundmaterialbywashing;5)bindingofstreptavidinconjugatedhorserADIshperoxidasetothebiotinylatedantibodies;6)removalofexcessorunboundenzymeconjugatesbywashing;and7)quantificationofimmobilizedantibody-enzyme complexesbymonitoringhorseradishperoxidaseactivityinthepresenceofthesubstrate3,3’,5,5’-tetramethylbenzidine(TMB).Enzymeactivityismeasuredspectrophotometricallybyrecordingabsorbancereadingsat450nm.Increasesinabsorbencyaredirectlyproportionaltotheamountof GFAPproteincapturedfromthesample.ThereforetheproteinconcentrationofunknownscanbederivedbyinterpolationusingastandardcurvegeneratedinthesameassaywithreferencestandardsofknownconcentrationsofGFAP.
AlternateNames	GFAP GlialFibrillaryAcidicProteinELISA
MaterialsRequiredbutNotDelivered	1.Absorbentpaperorcloth 2.Conventional,multi-channel,orrepeatingPipettes 3.Reagentreservoirs 4.Orbitalmicrotiterplateshaker/washer(optional) 5.GraduatedSEROlogicalpipettes 6.Microtiterplatereadercapableofreadingabsorbencyat450nm 7.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 8.Microfugetubesforstandardandsampledilutions 9.Mechanicalvortex 10.Milli-Q?Water

ProductInformation
Components	96-WellAnti-GFAPCoatedPlate(CS206352):1Plate 1XAssayBuffer(CS206357):40mL 10XWashBuffer(CS206360):50mL GFAPProteinStandard(CS206361):1Vial GFAPQualityControl#1(CS206351):1Vial GFAPQualityControl#2(CS206475):1Vial BiotinylatedAnti-GFAPDetectionAntibody(CS206356):12mL EnzymeSolution(CS206358):12mL TMBSolution(CS202682):10mL StopSolution(60193):12mL PlateSealers(CS200443):10Sheets
Detectionmethod	Chromogenic

StorageandShippingInformation
StorageConditions	Storage:TheGFAPELISAkitisshippedoniceandshouldbestoredat2-8°Cuponarrival. StABIlity:Kitcomponentsarestableforaminimumof6monthsfromthedateofreceiptifstoredand handledasdescribedabove.

Applications
KeyApplications	ELISA

BiologicalInformation
SpeciesReactivity	Mouse Rat Human
AnalytesAvailable	GFAP
EntrezGeneNumber	NM_002055.2
EntrezGeneSummary	Thisgeneencodesoneofthemajorintermediatefilamentproteinsofmatureastrocytes.Itisusedasamarkertodistinguishastrocytesfromotherglialcellsduringdevelopment.MutationsinthisgenecauseAlexanderdisease,araredisorderofastrocytesinthecentralnervoussystem.Alternativesplicingresultsinmultipletranscriptvariantsencodingdistinctisoforms.
GeneSymbol	GFAP
UniProtNumber	P14136
UniProtSummary	FUNCTION:GFAP,aclass-IIIintermediatefilament,isacell-specificmarkerthat,duringthedevelopmentofthecentralnervoussystem,distinguishesastrocytesfromotherglialcells. SUBCELLULARLOCATION:Cytoplasm.Note:Associatedwithintermediatefilaments. TISSUESPECIFICITY:Expressedincellslackingfibronectin. PTM:PhosphorylatedbyPKN1. SIMILARITY:Belongstotheintermediatefilamentfamily.

PhysicochemicalInformation
Sensitivity	1.5ng/mL
StandardCurveRange	1.5to100ng/mL

Dimensions

MaterialsInformation

MaterialsInformation

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Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

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