Description:

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Peptide:N-GlycosidaseF,alsoknownasPNGaseF,isarecombinantamidasewhichcleavesbetweentheinnermostGlcNAcandasparagineresiduesofhighmannose,hybrid,andcomplexoligosaccharidesfromN-linkedglycoproteins(1).
ProductSource
ClonedfromElizabethkingiamiricola(formerlyFlavobacteriummeningosepticum)andexpressedinE.coli(2).Specificity

ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
|  | Storeat(°C) | Concentration | 
| GlycoproteinDenaturingBuffer | -20 | 10X | 
| GlycoBuffer2 | -20 | 10X | 
| NP-40 | -20 | 10% | 
Notes:
Todeglycosylateanativeglycoprotein,longerincubationtimeaswellasmoreenzymemayberequired.SincePNGaseF,RecombinantactivityisinhibitedbySDS,itisessentialtohaveNP-40inthereactionmixture.Itisnotknownwhythisnon-ionicdetergentcounteractstheSDSinhibitionatthepresenttime.PNGaseF,RecombinantwillnotcleaveN-linkedglycanscontainingcoreα1-3Fucose.