The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Cbl (Phospho-Tyr731) Antibody
Synonyms
E3 ubiquitin-protein ligase CBL; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human Cbl around the phosphorylation site of Tyr731.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Cbl (Phospho-Tyr731) antibody detects endogenous levels of Cbl only when phosphorylated at Tyr731.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CBL
Database Links
Gene ID: 867 Swiss-Prot #: P22681
OMIM #: 165360 Unigene #: Hs.504096
Target Modification
Phospho
Modification Sites
H:Y731, M:Y737, R:Y845
Application Images
Image 2
Western blot analysis of lysates from HeLa cells treated with EGF, using phospho-Cbl (Phospho-Tyr731) antibody.