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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Cytokeratin 8 (Phospho-Ser74) Antibody |
| Synonyms | Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8; CK-8 |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human Cytokeratin 8 around the phosphorylation site of Ser74. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:10000 |
| Specificity | Cytokeratin 8 (Phospho-Ser74) antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at Ser74. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | KRT8 |
| Database Links |
Gene ID: 3856 Swiss-Prot #: P05787 OMIM #: 148060/215600 Unigene #: Hs.533782/Hs.708445 |
| Target Modification | Phospho |
| Modification Sites | H:S74, M:S80 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cytokeratin 8 (Phospho-Ser74) Antibody |
| Image 2 |
 Western blot analysis of lysates from 293 cells treated with UV, using p-Cytokeratin 8 (Phospho-Ser74) antibody. |
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