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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
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| Product Name | HSP27 (Phospho-Ser78/Ser82) Antibody |
| Synonyms | 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27 |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human HSP27 around the phosphorylation site of Ser78/Ser82. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | IHC: 1:50~1:100 ELISA: 1:10000 |
| Specificity | HSP27 (Phospho-Ser78/S82) antibody detects endogenous levels of HSP27 only when phosphorylated at Ser78/Ser82. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | HSPB1 |
| Database Links |
Gene ID: 3315 Swiss-Prot #: P04792 OMIM #: 602195/606595/608634 Unigene #: Hs.520973 |
| Target Modification | Phospho |
| Modification Sites | H:S78+S82 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP27 (Phospho-Ser78/S82) Antibody |
| Image 3 |
 Immunohistochemistry analyzsis of phospho-HSP27 (Phospho-Ser78/82) antibody in paraffin-embedded human breast carcinoma tissue. |
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