The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
p130 Cas (Phospho-Tyr128) Antibody
Synonyms
Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas; BCAR1; CAS; CASS1; CRKAS
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human p130 Cas around the phosphorylation site of Tyr128.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
p130 Cas (Phospho-Tyr128) antibody detects endogenous levels of p130 Cas only when phosphorylated at Tyr128.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BCAR1
Database Links
Gene ID: 9564 Swiss-Prot #: P56945
OMIM #: 602941 Unigene #: Hs.479747
Target Modification
Phospho
Modification Sites
H:Y128
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p130 Cas (Phospho-Tyr128) Antibody
Image 2
Western blot analysis of lysates from HepG2 cells treated with EGF, using phospho-p130 Cas (Phospho-Tyr128) antibody.