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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | MARCKS (Phospho-Ser158) Antibody |
| Synonyms | 80K-L protein; MACS; MARCS; myristoylated alanine-rich C-kinase substrate; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human MARCKS around the phosphorylation site of Ser158. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:10000 |
| Specificity | MARCKS (Phospho-Ser158) Antibody detects endogenous levels of MARCKS only when phosphorylated at Ser158. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | MARCKS |
| Database Links |
Gene ID: 4082 Swiss-Prot #: P29966 OMIM #: 177061 Unigene #: Hs.519909/Hs.712721 |
| Target Modification | Phospho |
| Modification Sites | H:S159, M:S152, R:S152 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MARCKS (Phospho-Ser158) Antibody |
| Image 2 |
 Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using MARCKS (Phospho-Ser158) Antibody. |
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