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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Retinoblastoma (Phospho-Thr826) Antibody |
| Synonyms | P105-RB; PP105; PP110; RB-1; RB1; retinoblastoma gene; Retinoblastoma-associated protein |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human Retinoblastoma around the phosphorylation site of Thr826. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:10000 |
| Specificity | Retinoblastoma (Phospho-Thr826) Antibody detects endogenous levels of RB1 only when phosphorylated at Thr826. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | RB1 |
| Database Links |
Gene ID: 5925 Swiss-Prot #: P06400 OMIM #: 109800/180200/259500 Unigene #: Hs.408528 |
| Target Modification | Phospho |
| Modification Sites | H:T826, M:T819, R:T797 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Retinoblastoma (Phospho-Thr826) Antibody |
| Image 2 |
 Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using Retinoblastoma (Phospho-Thr826) Antibody. |
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