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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | SAPK/JNK (Phospho-Tyr185) Antibody |
| Synonyms | JNK-46; JNK1-alpha-2; MAPK8; MK08; Mitogen-activated protein kinase 8; PRKM8; SAPK gamma; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; kinase JNK1; p54 gamma |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
| Specificity | SAPK/JNK (Phospho-Tyr185) Antibody detects endogenous levels of MAPK8/9/10 only when phosphorylated at Tyr185. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | MAPK8/9/10 |
| Database Links |
Gene ID: 5599/5601/5602 Swiss-Prot #: P45983/P45984/P53779 OMIM #: 601158/602896/602897/606369 Unigene #: Hs.138211/Hs.654461/Hs.125503 |
| Target Modification | Phospho |
| Modification Sites | H:Y185/223, M:Y185/223, R:Y185/223 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SAPK/JNK (Phospho-Tyr185) Antibody |
| Image 2 |
 Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using SAPK/JNK (Phospho-Tyr185) Antibody. |
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