The antiserum was produced against synthesized phospho-peptide derived from human SREBP-1 around the phosphorylation site of Ser439.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
SREBP-1 (Phospho-Ser439) Antibody detects endogenous levels of SREBF1 only when phosphorylated at Ser439.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SREBF1
Database Links
Gene ID: 6720 Swiss-Prot #: P36956
OMIM #: 184756 Unigene #: Hs.592123/Hs.655395
Target Modification
Phospho
Modification Sites
H:S439, R:S432
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SREBP-1 (Phospho-Ser439) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30', using SREBP-1 (Phospho-Ser439) Antibody.