Sigma-Aldrich/Protease from Bacillus licheniformis/P3910-500MG/500MG

• CAS Number 9014-01-1

•  EC Number 232-752-2

•  Enzyme Commission (EC) Number 3.4.21.62  ( BRENDA | IUBMB )

•  MDL number MFCD00132092

•  eCl@ss 32160410

• Popular Documents:  Specification Sheet (PDF)  

Properties:

Description:

Packaging

500 mg in poly bottle

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Protocols & Applications

Non-specific Protease Activity Assay Video

Safety:

Symbol 

  GHS05, GHS07, GHS08, GHS09