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Millipore/S7804 | CpG WIZ? E-Cadherin Amplification Kit/S7804/25 assays

價格: ￥6228.00 市場價: 10380.00

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貨號: S7804

品牌: Millipore

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Description
CatalogueNumber	S7804
BrandFamily	Chemicon®
TradeName	CpGWiz Chemicon
Description	CpGWIZ?E-CadherinAmplificationKit
Overview	E-cadherinisacelladhesionproteinthatsuppressestumorcellinvasionandmetastasisintumormodels.OnmechanismregulatingtheexpressionoftheE-cadheringeneismethylationofthepromoterregion.TheCpGWIZ?E-cahedrinAmplificationKitisusedfordeterminingthemethylationofstatusoftheE-cadherinpromoterbymethylation-specificPCR(MSP).Thekitcontainsprimerstargetedtoregionsofthepromoterwherethesequencesaremostdivergentafterbisulfitetreatment.PCRparametershavebeenidentifiedsothatallprimersetsinthekitamplifyunderthesameconditions.ControlgenomicDNAsamples(methylatedandunmethylated)forE-cadherinarealsoincluded. Methylation-specificPCR(MSP)isanewtechnologyforsensitivedetectionofabnormalgenemethylationutilizingsmallamountsofDNA(Herman,1996).ThisprocessemploysaninitialbisulfitereactiontomodifytheDNA,followedbyPCRamplificationwithspecificprimersdesignedtodistinguishmethylatedfromunmethylatedDNA.
AlternateNames	CD324

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Applications
Application	TheCpGWIZE-cahedrinAmplificationKitisusedfordeterminingthemethylationofstatusoftheE-cadherinpromoterbymethylation-specificPCR(MSP).
KeyApplications	PCR
ApplicationNotes	PrinciplesoftheTechnique UseofeithertheCpGenome?DNAModificationKit(Cat.No.S7820)ortheCpGENOME?FastDNAModificationKit(Cat.No.S7824)facilitatestheinitialbisulfitereactions,whiletheCpGWIZ?E-cadherinAmplificationKitcontainsthereagentsrequiredforthegene-specificPCRamplificationreactions.

BIOLOGicalInformation
SpeciesReactivity	Human
EntrezGeneNumber	NM_004360.2
EntrezGeneSummary	Thisgeneisaclassicalcadherinfromthecadherinsuperfamily.Theencodedproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Mutationsinthisgenearecorrelatedwithgastric,breast,colorectal,thyroidandovariancancer.Lossoffunctionisthoughttocontributetoprogressionincancerbyincreasingproliferation,invasion,and/ormetastasis.Theectodomainofthisproteinmediatesbacterialadhesiontomammaliancellsandthecytoplasmicdomainisrequiredforinternalization.Identifiedtranscriptvariantsarisefrommutationatconsensussplicesites.
GeneSymbol	CDH1 CDHE E-cadherin Arc-1 Uvomorulin UVO Cadherin-1 CD324 uvomorulin ECAD LCAM
UniProtNumber	P12830
UniProtSummary	FUNCTION:SwissProt:P12830#E-Cad/CTF2promotesnon-amyloidogenicdegradationofAbetaprecursors.HasastronginhibitoryeffectonAPPC99andC83production. SIZE:882aminoacids;97456Da SUBUNIT:Homodimer;disulfide-linked.Interactsdirectly,viathecytoplasmicdomain,withCTNNB1orJUPtoformthePSEN1/cadherin/cateninadhesioncomplexwhichconnectstotheactinskeletonthroughtheactinbindingofalpha-catenin.InteractionwithPSEN1,cleavesCDH1resultinginthedisassociationofcadherin-basedadherensjunctions(CAJs).InteractswithAJAP1,CTNND1andDLG7. SUBCELLULARLOCATION:Celljunction.Cellmembrane;Single-passtypeImembraneprotein.Note=ColocalizeswithDLG7atsitesofcell-cellcontactinintestinalepithelialcells.AnchoredtoactinMICROFILamentsthroughassociationwithalpha-,beta-andgamma-catenin.Sequentialproteolysisinducedbyapoptosisorcalciuminflux,resultsintranslocationfromsitesofcell-cellcontacttothecytoplasm. TISSUESPECIFICITY:Non-neuralepithelialtissues. PTM:Duringapoptosisorwithcalciuminflux,cleavedbyamembrane-boundmetalloproteinase(ADAM10),PS1/gamma-secretaseandcaspase-3toproducefragmentsofabout38kDa(E-CAD/CTF1),33kDa(E-CAD/CTF2)and29kDa(E-CAD/CTF3),respectively.Processingbythemetalloproteinase,inducedbycalciuminflux,causesdisruptionofcell-celladhesionandthesubsequentreleaseofbeta-cateninintothecytoplasm.Theresidualmembrane-tetheredcleavageproductisrapidlydegradedviaanintracellularproteolyticpathway.Cleavagebycaspase-3releasesthecytoplasmictailresultingindisintegrationoftheactinmicrofilamentsystem.Thegamma-secretase-mediatedcleavagepromotesdisaaaemblyofadherensjunctions. DISEASE:"SwissProt:P12830#DefectsinCDH1areinvolvedindysfunctionofthecell-celladhesionsystem,triggeringcancerinvasion(gastric,breast,ovary,endometriumandthyroid)andmetastasis.&DefectsinCDH1areacauseofhereditarydiffusegastriccancer(HDGC)[MIM:137215].&DefectsinCDH1areacauseofsusceptibilitytoendometrialcancer[MIM:608089]." SIMILARITY:SwissProt:P12830##Contains5cadherindomains.

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Millipore/S7804 | CpG WIZ? E-Cadherin Amplification Kit/S7804/25 assays

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