Certified liquid Hellma® calibration standard to enable the checking of spectrophotometers with respect to stray light.
Consisting of : Potassium chloride in purified water (667-UV1, wavelength 200 nm [Cut-Off]) Purified water reference (667-UV12, wavelengths 198; 200; 300; 400 nm [certified])
These calibration standards show a remarkably high absorbance below a particular wavelength (cut-off wavelength) in the UV range. Using this phenomenon, the spectrophotometer can be checked for stray radiation. The measurement of stray light is carried out against a reference filter containing ultra purified water, which is supplied with every set.
The spectrophotometer is set to a wavelength which is about 30 nm higher than the cut-off wavelength of the stray light filter being used (i.e. 230 nm for potassium chloride). Then scan down into the short wave region and observe the absorbance values below the cut-off wavelength. The stray light value for the measuring device results from the difference between the maximum measurable absorbance and the displayed absorbance.
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